Assay Principle

Principle

HMGB1 ELISA Kit II quantifies HMGB1 by sandwich ELISA. 10 µL specimen is added to immobilized anti-HMGB1 antibody on the well together with 100 µL sample diluent, allowing HMGB1 to specifically bind to the antibody. Peroxidase (POD)-conjugated secondary antibody is then added to the sample well. Chromogenic solution is added to the antigen-antibody complex for assaying absorbacen at 450 nm.

Assay Outline

HMGB1 ELISA KitⅡ
Add 100 µL Sample Diluent to each well.
Add 10 µL samples to each well.
↓ Incubate for 20-24 hours at 37°C
Wash wells 5 times.
Add 100µL POD-conjugate solution to each well.
↓ Incubate for 2 hours at 25°C
Wash wells 5 times.
Add 100µL substrate solution to each well.
↓ Incubate for 30 minutes at room temperature.
Add 100µL stop solution to each well.
Read at 450 nm.

Contact Us

For all sales and technical inquiries, please contact us via contact form or send us e-mails to the address shown below.

International Sales and Marketing
TEL: +81-42-718-5200
E-mail:HMGB_info@shino-test.co.jp
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For sales inquiries outside Japan, China (including Hong Kong and Macau) and Taiwan, please contact
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